Salon transcripts

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Collection. Shrimp samples were collected at stages of nauplius (N), zoea (Z), mysis (M) and 15-day-old postlarva (PL15) for transcriptome analysis.Full size imageFigure 2Summary of assembly and annotation of P. monodon at early developmental stages, including nauplius, zoea, mysis and 15-day-old postlarva. (a) Summary of read depth of RNAseq data. (b) The pie chart shows percentage of transcripts matched sequences in NCBI RefSeq database. (c) Histogram gene ontology classification of the transcripts into three main categories including biological process, molecular function and cellular component.Full size imageClustering of gene expression profiles across shrimp growth stages and enrichment analysisTo determine the gene expression dynamics in the early life of P. monodon, gene expression clustering was performed to categorize expression patterns associated with the developmental stages (Fig. S1). Our gene enrichment analysis revealed transcripts with their function in various biological processes (i.e., signal transduction, cell cycle, RNA polymerase II transcription, post-translational protein modification), metabolic pathways (i.e., metabolisms, metabolism of proteins and metabolism of carbohydrates) and developmental biological pathways and immune system in all stages. Gene expression patterns were grouped into 9 clusters. Cluster 1 to 4 showed highly expressed genes in each life stage, namely nauplius (1062 transcripts), zoea (71 transcripts), mysis (139 transcripts) and PL15 (1,146 transcripts). Cluster 5 (2554 transcripts) and 6 (423 transcripts) showed decreasing gene expression patterns with developmental stages, while cluster 7 (3780 transcripts) and 8 (1237 transcripts) showed increasing expression patterns from the nauplius stage to the later growth stage. Cluster 9 (9870 transcripts) was a group of transcripts with unchanged expression levels throughout early developmental stages.Expression profiles of genes related to metabolic pathways and growth development in early life stages of P. monodon Here, expression levels of transcript-related to metabolic processes, and cell and growth development were identified in larval stages (Fig. 3 and Table S1).

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In a side panel with timestamps.You can adjust the frequency of timestamps in the plugin settings.Clicking on a timestamp will open the video at that specific point.You can drag and drop lines from the transcript into your notes as needed.For more information and updates, you can visit the plugin’s GitHub repository. citeturn0search8By following these steps, you can seamlessly integrate YouTube video transcripts into your Obsidian workflow.​YTranscript is a free Chrome extension designed to enhance your YouTube experience by allowing you to generate and search video transcripts directly from the sidebar. With just a click, you can instantly access transcripts for any YouTube video, enabling you to:Search Within Transcripts: Quickly locate specific information without manually sifting through the entire video.Skip to Key Moments: Save time by jumping directly to important sections in lengthy videos.Boost Research and Study Sessions: Easily access spoken content, making note-taking and content review more efficient.This tool is particularly beneficial for students conducting research, professionals seeking precise information, or anyone looking to maximize their YouTube viewing efficiency. By eliminating the need for tedious note-taking or constant rewinding, YTranscript streamlines your interaction with video content.To get started, you can add YTranscript to your Chrome browser from the Chrome Web Store. citeturn0search0If you’re interested in alternative tools for extracting YouTube video transcripts, here are a few options:YouTube Transcript: Offers instant transcripts and supports multiple languages without requiring sign-up. citeturn0search7YouTube to Transcript: Allows users to extract transcripts and provides features like translation and embedded video playback with synchronized transcripts. citeturn0search2YouTube Transcript Generator by NoteGPT: Provides free online transcription with AI summarization capabilities and cloud storage for notes. citeturn0search11These tools offer various features to help you extract and interact with transcripts from YouTube videos, catering to different preferences and needs.

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A group of transcripts was highly expressed and specific to each larval shrimp stage (Fig. 3a). For instance, the transcripts involved in cell and growth development such as Krüppel-like factor, zinc finger protein, histone and homeobox protein were enriched in the nauplius stage (Cluster 1), while alpha (1,6)-fucosyltransferase was highly expressed in the zoea stage (Cluster 2). Moreover, the PL15 stage (Cluster 4) showed increased expression levels of actin, ferritin and tubulin transcripts. On the other hand, transcripts-related with metabolic pathways such as galactoside alpha-(1,2)-fucosyltransferase in zoea, cAMP-dependent protein kinase catalytic and NADH dehydrogenase in mysis (Cluster 3), and carbohydrate sulfotransferase in PL15 stage. These results show that the dynamic changes of distinct classes of genes during the development reflecting different needs of gene functions for their metabolic processes and maintenance of their cellular functions.Figure 3Heatmap showing the enriched transcripts involved in metabolic processes and cell and growth development associated with early shrimp stages, which were divided to nauplius (N), zoea (Z), mysis (M) and 15-day-old postlarva (PL15). (a) Cluster 1–4 shows the pattern of highly gene expressed profiles in nauplius, zoea, mysis and 15-day-old postlarva. (b) Cluster 5–6 shows decreasing pattern of gene expression from nauplius to postlarval stage, (c) cluster 7–8 shows increasing pattern of gene expression from nauplius to postlarval stage, and (d) cluster 9 shows the pattern of unchanged in gene expression across all life stages.Full size imageTo further explore changes in metabolisms and growth-related pathways under shrimp metamorphosis, the transcripts with continuously decreasing expression patterns (Cluster 5) were mostly members of a ribosomal protein family (Fig. 3b). The higher numbers of various components of ribosomal protein transcripts were found in nauplii, suggesting different physiological and metabolic activities in each shrimp’s life stage. Moreover, transcripts encoding cyclins and zinc finger proteins which play important roles in. Salon Transcripts S. Salon Transcripts CLAIM THIS BUSINESS. 2500 W HIGGINS RD STE 250 HOFFMAN ESTATES, IL Get Directions (800) . www.salontranscripts.com .

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